Eastern Anatolia, southern Iraq, and Jerusalem have been proposed as the regions that once contained the Garden of Eden. Several creationists have argued that it is impossible to locate the region of Eden due to the dramatic changes to the surface of the earth during the Noachian Deluge. However, a close analysis of relevant Biblical passages and the archaeology, geography, geology, paleontology, and paleobotany of Anatolia, the Near East, and North Africa suggest that the region of Eden was located in Southeastern Anatolia. This region provides source waters for four rivers, following Precambrian rift valleys or faults, which are connected to the Biblical locales of Asshur, Havilah, and Cush. The rivers traversed a landmass that originated in the Precambrian not covered by the alleged expanse of the Tethys Ocean. Furthermore, southeastern Anatolia is associated with the ancient kingdom of the House of Eden. Although the Bible states that the Flood was a global catastrophic event, the confluence of biblical texts with geographical, geological, and other data provides a compelling indication that its effects did not eradicate all evidence of Eden’s original location.