Biostratigraphy is a methodology for determining relative timing of sedimentary deposits based on their fossil content. It foundationally assumes faunal succession—that fossil ordering arises because different organisms lived in different eras of history. The Genesis Flood violates this assumption with all fossilized forms existing simultaneously at the beginning of the Flood. To date, no one has simulated the fossil record to test the effectiveness of biostratigraphic methods on the Flood-deposited fossil record when the fundamental assumptions have been transgressed. Fossil records with different ordering styles (true order, process order, successive inundation, endemism, and random), degree of ordering, and per-column fossil density were simulated, then the resulting timescales were statistically compared. The results of the simulations show that biostratigraphy consistently recovers the correct ordering of the fossil record when one exists. However, the method will also reliably produce a self-consistent global ordering even in cases where one does not exist, leading to timing distortions between distant columns. Higher precision in the interpreted biostratigraphic timescale increases time distortions. This timing distortion comes from having only a small number of index assemblage permutations preserved in direct relationship with each other in particular locations. When such combinatorial completeness is low, as in the real fossil record, then global consistency of observed fossil superposition cannot distinguish between a highly ordered and unordered fossil record. This finding has the potential to change our understanding of the timing and relationships between geological events during the Flood, and impacts the way we interpret many types of geological and fossil data. It also may cause a one-to-many relationship between a single time event such as the post-Flood boundary and the international chronostratigraphic timescale. The experiments also show correlation between fossil range extensions and timing distortions. Radiometric methods, global isochronous events (for example, impacts, megasequence boundaries), distant fossil-strata correspondences, and stratomorphic series were considered as independent lines of evidence for relative timing. Currently, these chronometers support coarse ordering consistent with the various creationist proposals for generation of fossil ordering during the Flood, but cannot distinguish between them. Further research in these areas may be able to test for more fine-grained precision in fossil ordering.