This article explores the perspectives of the renowned American author Mark Twain (Samuel Langhorne Clemens) on the topics of creation and evolution during a time when evolutionary thought was gaining prominence. It compiles and documents numerous quotes from Twain's extensive writings that touch upon biblical history, creation, and Darwin's theory, revealing his humorous yet often critical views on both scientific and religious explanations of origins. The author analyzes Twain's skepticism towards scientific pronouncements, his familiarity with the Bible, and his later-life hostility towards organized religion and the Creator, ultimately suggesting that despite his wit, Twain's later bitterness led him away from satisfying answers about life's meaning found in Scripture. The article aims to highlight Twain's entertaining critiques, the historical skepticism surrounding early scientific ideas, and the enduring tension between creation and evolutionary viewpoints.